| 1 |
EG |
Download |
| Egypt J Pediatr Allergy Immunol |
|
2010 |
; |
8 |
( |
01 |
) |
: |
35-40 |
- |
|
|
, |
ESP - 49 |
|
|
|
| Apoptosis and mitochondrial membrane potential changes of T
lymphocytes from children with Down’s syndrome |
| Nagla Hassan Abu Faddan |
|
Douaa Sayed |
|
Nahla Al-Sharkawy |
|
|
|
|
|
|
|
|
| Background: Down's syndrome (DS) patients present a high risk of
developing alterations of the immune system. The increased susceptibility to
bacterial and viral infection, hematologic malignancies and autoimmune
disease, suggest that immunodeficiency is an integral part of DS. Little is
known about the mitochondrial damage and tendency to apoptosis in
peripheral T lymphocyte cells in DS individuals.
Objective: to evaluate the tendency to apoptosis and mitochondrial
membrane potential (??m) changes in peripheral T lymphocytes of DS
children both in the presence and in the absence of acute infection.
Patients and methods: The present study included thirty children had DS
(all of them trisomy 21 of nondisjunction type), and thirty normal children,
fifteen of each group had no evidence of acute infection and fifteen had
acute infection. Potential apoptosis was measured by flow cytometry using
annexin V. The ??m was assessed by the retention of Rhodamine 123.
Results: There was no significant difference in the percentage of CD3+ cells
or potential apoptotic T lymphocytes between DS children and controls
either in presence or in absence of acute infection. However, there was a
significant decrease in ??m in the peripheral T lymohocytes of DS children
when compared to the controls.
Conclusion: The function of T cells not their number is the main mechanism
responsible for the impairment of the immune system in DS children. T
lymphocytes in peripheral blood from DS patients do not display an
increased tendency to undergo apoptosis although a significant loss of ??m
was found. |
| |