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EG |
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| Egypt J Pediatr Allergy Immunol |
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2010 |
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8 |
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9-18 |
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ESP - 46 |
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| IL-13 R130Q single nucleotide polymorphism in asthmatic Egyptian
children |
| Zeinab A. El-Sayed |
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Shereen S. El-Sayed |
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Shereen B. El-Sayed |
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Sherif G. Eladawy |
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| Background: Asthma and its associated phenotypes are under a substantial
degree of genetic control. The common variant IL-13 gene polymorphism
R130Q is reported to be associated with the risk of development of asthma in
some populations.
Objective: We sought to study the association of IL-13 genetic variant
R130Q with bronchial asthma in Egyptian children and its relation to
various clinical and laboratory phenotypes of the disease.
Methods: IL13 gene polymorphism (R130Q) was detected by PCR
amplification followed by sequencing using pure script total DNA in 20
asthmatic patients in acute exacerbation. The results were compared to 20
healthy age and sex matched children.
Results: Asthmatic children had significantly higher frequency of
distribution of R130Q genotype (50%) than controls (15%). The serum total
IgE as percent of high normal for age was significantly higher in asthmatic
patients as compared to controls with a mean of 208.77 ±237.06% and
14.21 ± 8.08% respectively. No significant difference was observed in the
mean AEC(as a percent of high normal for age) of both groups (80.85 ±
116.4% and 82.50 ± 81.4% respectively). No significant differences were
observed between patients with IL-13 polymorphism R130Q and those
without such polymorphism as regards family history, relation of
exacerbations to upper respiratory tract infections, history of food allergy or
asthma grading. Serum total IgE was significantly higher in asthmatics with
GA genotype as compared to those with GG genotype with a mean of 373.25
± 238.11% and 44.28 ± 42.65% respectively. A similar finding was also
observed among the control group with a mean of 28.03 ± 9.12% and 11.77
± 5.00% respectively. Finally a significantly higher AEC was observed in
controls with GA as compared to GG genotype with a mean of 250.00 ±
51.96% versus 52.94 ± 36.87% respectively.
Conclusion: The common variant IL-13 gene polymorphism R130Q is
frequently associated with pediatric asthma. This variant is more active than
the wild type in inducing allergic inflammation as reflected by the higher
serum total IgE and AEC. Hence, IL-13R130Q may be candidate for future
gene therapy targeted at reducing the ill-effects of this polymorphism. |
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