Egypt J Pediatr Allergy Immunol, (October 2017), Volume No. 15, Issue 02  
Egypt J Pediatr Allergy Immunol 2017 ; 15 ( 02 ) : 63 - 68
, ESP - 239  
Original articles
Lymphocyte subtype dysregulation in a group of children with simple obesity
Hoda L. Elsayed,   Yasmin G. El Gendy   Nesrine M. Radwan   Botheina A. Farweez   Shimaa Fouda    
Background: Obesity as a global public health problem is increasing in prevalence. Reports showed that obese children are more liable to infection than lean ones; it was claimed that obese subjects have altered peripheral blood total lymphocyte counts in addition to reduced lymphocyte proliferative response to mitogen stimulation as well as dysregulated cytokine expression. Objective: This study aimed to evaluate the effect of childhood obesity on cell-mediated immunity as indicated by peripheral blood lymphocyte phenotyping. Methods: We enrolled 30 school-aged children (mean age 10±3.27 years). They comprised two groups; 20 obese children with a mean body mass index (BMI) of 39.2± 12.5 and 10 matched control subjects with mean BMI of 18.4± 1.9. They were subjected to detailed anthropometric evaluation including weight, height, and waist-hip ratio in addition to calculation of BMI, complete blood counting, and flow cytometric assessment of T-helper (CD4), T-cytotoxic/suppressor (CD8), and natural killer (CD56) cell counts. Results: The absolute lymphocyte (CD3) and natural killer cell (CD56) counts were comparable in both groups. However, the CD4%, CD8%, CD4/CD8 ratio were significantly lower in the obese children (p=0.02, 0.03, 0.015 respectively). A significant negative correlation could be elicited between the CD4 count and body weight, BMI, and a hip-waist ratio (p = 0.00); the same was observed for the CD4/CD8 ratio (p = 0.00). On the contrary, CD8 correlated positively to the body weight, BMI, and waist-hip ratio (p = 0.00 for each). Conclusion: Obesity has an impact on lymphocytic subset counts and further studies are needed to assess its effect on their function.